Cellular uptake of cobalamin: Transcobalamin and the TCblR/CD320 receptor
نویسندگان
چکیده
منابع مشابه
The protein and the gene encoding the receptor for the cellular uptake of transcobalamin-bound cobalamin.
The transcobalamin (TC, TCII) receptor (TCblR) on the plasma membrane binds TC- cobalamin (Cbl) and internalizes the complex by endocytosis. This receptor was purified from human placental membranes by affinity chromatography. Tryptic digest of the protein extracted from a sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel and subjected to liquid chromatography/mass spectrometry iden...
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To determine the nature of binding of transcobalamin II (TC-lI) to liver cells, we covalently coupled purified holoTC-ll to submicron latex minibeads using glutaraldehyde. Incubation of the probe with liver cell suspensions at 4 C led to its binding by endothelial cells but not by hepatocytes or Kupffer cells, as visualized by scanning electron microscopy. At 37 C, the probe was internalized by...
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Herein we report the synthesis of new water-soluble vitamin B12 prodrugs bearing metal complexes at the β-upper side of the cobalt center. A total of three derivatives with the general design {Co-C[triple bond, length as m-dash]C-bpy-M}, where M represents a cytotoxic metal complex, were prepared and tested for their cytotoxicity against MCF-7 breast cancer cells. The choice of the metal was or...
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BACKGROUND Low cobalamin concentrations are common, but their causes are often unknown. Transcobalamin I/haptocorrin (TC I/HC) deficiency, viewed as a rare cause, has not been examined systematically in patients with unexplained low serum cobalamin. METHODS Total TC I/HC was measured by RIA in three subgroups of 367, 160, and 38 patients with different categories of low cobalamin concentratio...
متن کاملA sex difference in serum cobalamin and transcobalamin IeveIs13
In healthy young adults, serum levels of cobalamin, unsaturated and total cobalamin binding capacity, and transcobalamin (TC) II are significantly higher in females, while TC III is higher in males. These findings have relevance to the reference values in normals for these tests. Am J Clin Nutr 1985;4l:784-786.
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ژورنال
عنوان ژورنال: Biochimie
سال: 2013
ISSN: 0300-9084
DOI: 10.1016/j.biochi.2013.02.004